dCas9-DNMT3L
(Plasmid #126588)

• Purpose: DNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
• Depositing Lab: David Segal
• Publication: O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):26. doi: 10.1186/s13072-019-0275-8.

Backbone

• Vector backbone: pcDNA3.3-TOPO
• Backbone size w/o insert (bp): 5407
• Total vector size (bp): 11080
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DNMT3L
• Alt name: DNA methyltransferase 3 like
• Species: H. sapiens (human)
• GenBank ID: NM_175867.2
• Entrez Gene: DNMT3L
• Tag / Fusion Protein:
  • 3XFLag-NLS-dCas9-DNMT3L-NLS

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: polyA TK R

Resource Information

• Supplemental Documents:
  • dCas9-DNMT3L.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry