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SHIP2 WT pBABE-His-Xpress
(Plasmid #126584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE Puro
  • Backbone size w/o insert (bp) 5169
  • Total vector size (bp) 9662
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SHIP2
  • Alt name
    SHIP2; 51C protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4400
  • GenBank ID
    Y14385.1 NM_001567
  • Entrez Gene
    INPPL1 (a.k.a. OPSMD, SHIP2)
  • Tag / Fusion Protein
    • His Xpress (N-terminal)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer cagcttcaataaccctgcc
  • 3′ sequencing primer gaaagtgctggctggtga
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SHIP2 WT pBABE-His-Xpress was a gift from Christophe Erneux (Addgene plasmid # 126584 ; http://n2t.net/addgene:126584 ; RRID:Addgene_126584)

  • For your References section:

    SHIP2 controls PtdIns(3,4,5)P(3) levels and PKB activity in response to oxidative stress. Zhang J, Liu Z, Rasschaert J, Blero D, Deneubourg L, Schurmans S, Erneux C, Pesesse X. Cell Signal. 2007 Oct;19(10):2194-200. doi: 10.1016/j.cellsig.2007.06.022. Epub 2007 Jun 30. 10.1016/j.cellsig.2007.06.022 PubMed 17643961
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