pXf20pemIK
(Plasmid
#126577)
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Purpose(Empty Backbone) Vector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126577 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR2.1
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Backbone manufacturerInvitrogen
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Modifications to backboneAddition of replication origin and pemI/pemK stability factor from Xylella fastidiosa
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXf20pemIK was a gift from Lindsey Burbank & Drake Stenger (Addgene plasmid # 126577 ; http://n2t.net/addgene:126577 ; RRID:Addgene_126577) -
For your References section:
Plasmid Vectors for Xylella fastidiosa Utilizing a Toxin-Antitoxin System for Stability in the Absence of Antibiotic Selection. Burbank LP, Stenger DC. Phytopathology. 2016 Aug;106(8):928-36. doi: 10.1094/PHYTO-02-16-0097-R. Epub 2016 Jun 7. 10.1094/PHYTO-02-16-0097-R PubMed 27088393
Map uploaded by the depositor.
