pBEAST2-sfGFP
(Plasmid #126575)

• Purpose: Strong constitutive expression of super-folder GFP for cell-free expression
• Depositing Lab: Jerome Bonnet
• Publication: Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi: 10.1038/s41467-019-09722-9.

Backbone

• Vector backbone: pBEST-OR2-OR1-Pr-UTR1-deGFP-T500
• Modifications to backbone: Two 40bp spacers were added to the each end of the insert to facilitate Gibson assembly cloning into more complex constructs. Upstream and downstream terminators (B0014 and T500, respectively) were flanked by two 40bp spacers. The OR2-OR1-Pr promoter and UTR were conserved, but deGFP was replaced by sfGFP.
• Vector type: Synthetic Biology ; Cell-Free Protein Synthesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): KL740+
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sfGFP
• Species: Synthetic
• Insert Size (bp): 720

Resource Information

• Supplemental Documents:
  • pBEAST2-sfGFP.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The terminator B0015 from the pBEAST-sfGFP plasmid in the publication was replaced by T500 as the original strain was found to exhibit some recombination events.