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Addgene

pMT2 JNK3 p46
(Plasmid #12657)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12657 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT2
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JNK3
  • Alt name
    SAPK p46 beta1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1300
  • Entrez Gene
    Mapk10 (a.k.a. Jnk3, SAPKC, SAPb, Serk2)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer pMT2-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

p46 betaI has 5 bp insertion (AGCAC) at C-terminus. C-terminal amino acid sequence is QPSPSAQVQQ-stop.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT2 JNK3 p46 was a gift from John Kyriakis (Addgene plasmid # 12657 ; http://n2t.net/addgene:12657 ; RRID:Addgene_12657)
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