pME-GcAMP5
(Plasmid
#126569)
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PurposeMultisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126569 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
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Vector typeGateway entry clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGcAMP5
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-GcAMP5 was a gift from Rob Parton (Addgene plasmid # 126569 ; http://n2t.net/addgene:126569 ; RRID:Addgene_126569) -
For your References section:
Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle. Lo HP, Lim YW, Xiong Z, Martel N, Ferguson C, Ariotti N, Giacomotto J, Rae J, Floetenmeyer M, Moradi SV, Gao Y, Tillu VA, Xia D, Wang H, Rahnama S, Nixon SJ, Bastiani M, Day RD, Smith KA, Palpant NJ, Johnston WA, Alexandrov K, Collins BM, Hall TE, Parton RG. J Cell Biol. 2021 Dec 6;220(12). pii: 212693. doi: 10.1083/jcb.201905065. Epub 2021 Oct 11. 10.1083/jcb.201905065 PubMed 34633413