pBBR5pemIK
(Plasmid
#126554)
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Purpose(Empty Backbone) Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126554 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBBR1-MCS5
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Modifications to backbonepemI/pemK plasmid stability factor
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Vector typeBacterial Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBBR5pemIK was a gift from Lindsey Burbank & Drake Stenger (Addgene plasmid # 126554 ; http://n2t.net/addgene:126554 ; RRID:Addgene_126554) -
For your References section:
Plasmid Vectors for Xylella fastidiosa Utilizing a Toxin-Antitoxin System for Stability in the Absence of Antibiotic Selection. Burbank LP, Stenger DC. Phytopathology. 2016 Aug;106(8):928-36. doi: 10.1094/PHYTO-02-16-0097-R. Epub 2016 Jun 7. 10.1094/PHYTO-02-16-0097-R PubMed 27088393
Map uploaded by the depositor.
