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Addgene

pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef
(Plasmid #126553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 vector pNL4-3
  • Species
    HIV
  • Insert Size (bp)
    6251
  • GenBank ID
    NM_12831134
  • Promoter HIV LTR
  • Tag / Fusion Protein
    • Thy1.2 and GFP

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef was a gift from Jonathan Karn (Addgene plasmid # 126553 ; http://n2t.net/addgene:126553 ; RRID:Addgene_126553)

  • For your References section:

    Entry of Polarized Effector Cells into Quiescence Forces HIV Latency. Dobrowolski C, Valadkhan S, Graham AC, Shukla M, Ciuffi A, Telenti A, Karn J. MBio. 2019 Mar 26;10(2). pii: mBio.00337-19. doi: 10.1128/mBio.00337-19. 10.1128/mBio.00337-19 PubMed 30914509
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