pHR-H13LTat-CD8a/d2eGFP-IRES-Nef
(Plasmid
#126552)
-
PurposeReplication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepHR
-
Vector typeLentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHIV-1 vector pNL4-3
-
SpeciesHIV
-
Insert Size (bp)7228
-
MutationDelta gag/pol
-
GenBank IDNM_12831134
- Promoter HIV LTR
-
Tag
/ Fusion Protein
- CD8a and GFP
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EBV Reverse (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHR-H13LTat-CD8a/d2eGFP-IRES-Nef was a gift from Jonathan Karn (Addgene plasmid # 126552 ; http://n2t.net/addgene:126552 ; RRID:Addgene_126552) -
For your References section:
Entry of Polarized Effector Cells into Quiescence Forces HIV Latency. Dobrowolski C, Valadkhan S, Graham AC, Shukla M, Ciuffi A, Telenti A, Karn J. MBio. 2019 Mar 26;10(2). pii: mBio.00337-19. doi: 10.1128/mBio.00337-19. 10.1128/mBio.00337-19 PubMed 30914509
Map uploaded by the depositor.
