AAV-Flex-rev-GFPFANACfullWT
(Plasmid #126551)

• Purpose: AAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channel
• Depositing Lab: Rory McQuiston
• Publication: Schanuel et al Neuroscience. 2008 Aug 13;155(2):374-86. doi: 10.1016/j.neuroscience.2008.05.052. Epub 2008 Jun 10.

Backbone

• Vector backbone: AAV-FLEX-rev-ChR2-tdtomato
• Backbone manufacturer: Addgene plasmid 18917
• Backbone size w/o insert (bp): 5045
• Total vector size (bp): 7604
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): MAX Efficiency™ Stbl2™ Competent Cells (Thermo Fisher)
• Growth instructions: Grow at 30 degrees in 2xYT media (see comments section below for more information). Please use 20 ug/ml of Amp.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: eGFP N-terminal fused to FMRFamide gate sodium channel
• Alt name: GFPFANAC
• Species: Helix aspersa
• Insert Size (bp): 2600

Resource Information

• Supplemental Documents:
  • AAV-Flex-rev-GFPFANACfullWT.ape
• A portion of this plasmid was derived from a plasmid made by: Eric Lingueglia, Michel Lazdunski

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To minimize recombination, propagate this plasmids in Stbl2 cells. Also, to minimize recombination, cells should be cultured at 30 C.

Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.

For more information on growth conditions, please see www.addgene.org/18917 as this is the backbone vector. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. The expected patterns can be calculated from the attached sequence.