Skip to main content
Addgene

Ypd1p-pET22b
(Plasmid #12655)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12655 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET22b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YPD1 protein
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1003
  • GenBank ID
    Z74283
  • Entrez Gene
    YPD1 (a.k.a. YDL235C)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ypd1p-pET22b was a gift from SeWon Suh (Addgene plasmid # 12655 ; http://n2t.net/addgene:12655 ; RRID:Addgene_12655)

  • For your References section:

    Insights into eukaryotic multistep phosphorelay signal transduction revealed by the crystal structure of Ypd1p from Saccharomyces cerevisiae. Song HK, Lee JY, Lee MG, Moon J, Min K, Yang JK, Suh SW. J Mol Biol. 1999 Nov 5. 293(4):753-61. 10.1006/jmbi.1999.3215 PubMed 10543964
Related items: