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Addgene

FLAG-HA-RNF68 [dRING]
(Plasmid #126549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE
  • Backbone size w/o insert (bp) 5169
  • Total vector size (bp) 5903
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNF68
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    734
  • Mutation
    RING domain deletion mutant [dRING mutation is a deletion of AA 47-86]
  • GenBank ID
    NP_116062.2
  • Entrez Gene
    PCGF1 (a.k.a. 2010002K04Rik, NSPC1, RNF3A-2, RNF68)
  • Promoter LTR
  • Tag / Fusion Protein
    • FLAG-HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-HA-RNF68 [dRING] was a gift from Michele Pagano (Addgene plasmid # 126549 ; http://n2t.net/addgene:126549 ; RRID:Addgene_126549)

  • For your References section:

    PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading. Rona G, Roberti D, Yin Y, Pagan JK, Homer H, Sassani E, Zeke A, Busino L, Rothenberg E, Pagano M. Elife. 2018 Jul 9;7. pii: 38771. doi: 10.7554/eLife.38771. 10.7554/eLife.38771 PubMed 29985131
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