mCherry-RNF68
(Plasmid #126545)

• Purpose: Expresses mCherry-tagged RNF68 in mammalian cells
• Depositing Lab: Michele Pagano
• Publication: Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10.7554/eLife.38771.

Backbone

• Vector backbone: pBABE
• Backbone size w/o insert (bp): 7605
• Total vector size (bp): 8388
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RNF68
• Species: H. sapiens (human)
• Insert Size (bp): 783
• GenBank ID: NP_116062.2
• Entrez Gene: PCGF1 (a.k.a. 2010002K04Rik, NSPC1, RNF3A-2, RNF68)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: CMV_fwr
• 3′ sequencing primer: PGK_rev

Resource Information

• Supplemental Documents:
  • pretroq-mcherry-c1-with-rnf68.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry