GFP-FBXL11
(Plasmid #126543)

• Purpose: Expresses GFP-tagged FBXL11 in mammalian cells
• Depositing Lab: Michele Pagano
• Publication: Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10.7554/eLife.38771.

Backbone

• Vector backbone: pBABE
• Backbone size w/o insert (bp): 5169
• Total vector size (bp): 9379
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FBXL11
• Alt name: Lysine demethylase 2A
• Alt name: JHDM1A
• Species: H. sapiens (human)
• Insert Size (bp): 3490
• GenBank ID: NP_036440.1
• Entrez Gene: KDM2A (a.k.a. CXXC8, FBL11, FBL7, FBXL11, JHDM1A, LILINA)
• Promoter: LTR
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry