Integrase triple controller
(Plasmid
#126540)
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PurposePben-int5; Ptet-bxb1; Pbad-tp901: colE1 Chlo+
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126540 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneJ64100
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBenR_pBEN-int5_pTet-bxb1_AraC_pBAD-tp901-1
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Insert Size (bp)7771
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note there is a single mutation in arabinose V113A, and a single mutation in BxbI integrase E117G. Depositor confirms these do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Integrase triple controller was a gift from Jerome Bonnet (Addgene plasmid # 126540 ; http://n2t.net/addgene:126540 ; RRID:Addgene_126540) -
For your References section:
Rational programming of history-dependent logic in cellular populations. Zuniga A, Guiziou S, Mayonove P, Meriem ZB, Camacho M, Moreau V, Ciandrini L, Hersen P, Bonnet J. Nat Commun. 2020 Sep 21;11(1):4758. doi: 10.1038/s41467-020-18455-z. 10.1038/s41467-020-18455-z PubMed 32958811