pLenti.hUbC.H2B-CeruleanFP-2A-Dendra2FP.W
(Plasmid #126522)

• Purpose: Lentivector that expresses H2B-Cerulean and Dendra2 fluorescent proteins from an internal hUbC promoter.
• Depositing Lab: Rusty Lansford
• Publication: Huss et al Front Cell Dev Biol. 2019 Mar 28;7:35. doi: 10.3389/fcell.2019.00035. eCollection 2019.

Backbone

• Vector backbone: pLenti.CAG.H2B-CerFP-2A-Dendra2FP
• Backbone size w/o insert (bp): 9801
• Total vector size (bp): 9275
• Modifications to backbone: pLenti.CAG.H2B-CerFP-2A-Dendra2FP was digested with XhoI to remove CAG, Klenow blunted and gel isolated. The hUbC insert was PCR amplified from pFUGW using primers containing EcoRV RE sites, cut and gel isolated. Blunt end ligation was followed by RE analysis for directionality.
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: H2B-CeruleanFP-2A-Dendra2FP
• Alt name: Histone 2B Cerulean Fluorescent Protein-P2A-Dendra2 Fluorescent Protein
• Species: Synthetic
• Insert Size (bp): 1871
• Promoter: hUbC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: EGFP-N
• 3′ sequencing primer: EGFP-C

Resource Information

• Supplemental Documents:
  • pLenti hUbC H2B Cerulean 2A Dendra2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry