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Addgene

p-mCherry-C1-MerMAID4
(Plasmid #126516)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 3986
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MerMAID4
  • Species
    Synthetic
  • Insert Size (bp)
    1212
  • Promoter CMV (+ enhancer)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BshTI (not destroyed)
  • 5′ sequencing primer CMV-fwd: GCAAATGGGCGGTAGGCGT or EGFP-N1-fwd: GAGGTCTATATAAGCAGAGC
  • 3′ sequencing primer EGFP-C1-rev: AACCATTATAAGCTGCAATAAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-mCherry-C1-MerMAID4 was a gift from Peter Hegemann (Addgene plasmid # 126516 ; http://n2t.net/addgene:126516 ; RRID:Addgene_126516)

  • For your References section:

    MerMAIDs: a family of metagenomically discovered marine anion-conducting and intensely desensitizing channelrhodopsins. Oppermann J, Fischer P, Silapetere A, Liepe B, Rodriguez-Rozada S, Flores-Uribe J, Peter E, Keidel A, Vierock J, Kaufmann J, Broser M, Luck M, Bartl F, Hildebrandt P, Simon Wiegert J, Beja O, Hegemann P, Wietek J. Nat Commun. 2019 Jul 25;10(1):3315. doi: 10.1038/s41467-019-11322-6. 10.1038/s41467-019-11322-6 PubMed 31346176
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