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TM1442-pET-21a
(Plasmid #12650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5410
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Putative anti-sigma factor antagonist
  • Alt name
    TM1442
  • Species
    T. maritima
  • Insert Size (bp)
    333
  • Mutation
    N/A
  • GenBank ID
    AE000512
  • Entrez Gene
    TM1442

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TM1442-pET-21a was a gift from SeWon Suh (Addgene plasmid # 12650 ; http://n2t.net/addgene:12650 ; RRID:Addgene_12650)

  • For your References section:

    Crystal structure of the TM1442 protein from Thermotoga maritima, a homolog of the Bacillus subtilis general stress response anti-anti-sigma factor RsbV. Lee JY, Ahn HJ, Ha KS, Suh SW. Proteins. 2004 Jul 1. 56(1):176-9. 10.1002/prot.20166 PubMed 15162498
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