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Addgene

Ubiquitin I44A
(Plasmid #12648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin
  • Alt name
    Ubiquitin C
  • Alt name
    UBC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    228
  • Mutation
    Ile44Ala
  • GenBank ID
    Ubiquitin C
  • Entrez Gene
    UBC (a.k.a. HMG20)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ? (unknown if destroyed)
  • 3′ cloning site ? (unknown if destroyed)
  • 5′ sequencing primer universal T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ubiquitin I44A was a gift from Rachel Klevit (Addgene plasmid # 12648 ; http://n2t.net/addgene:12648 ; RRID:Addgene_12648)

  • For your References section:

    A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE. Mol Cell. 2006 Mar 17. 21(6):873-80. 10.1016/j.molcel.2006.02.008 PubMed 16543155
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