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pcDNA3.1(-)-mRho.V5.mER.hOr47a
(Plasmid #126472)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126472 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5427
  • Total vector size (bp) 6672
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Or47a
  • Alt name
    DmelOr47a
  • Alt name
    Odorant receptor 47a
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1251
  • Mutation
    codon optimization for H. sapiens
  • Entrez Gene
    Or47a (a.k.a. Dmel_CG13225, 47E.1, 47a, AN10, CG13225, DOR24, DOR47E.1, Dmel\CG13225, OR47a, Or24, Or47E.1, dor24, or47a)
  • Promoter CMV
  • Tags / Fusion Proteins
    • H. sapiens Rhodopsin 344QVAPA348 (mRho) (N terminal on insert)
    • V5 tag (N terminal on insert)
    • H. sapiens HCN1 106VNKFSL111 (mER) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(-)-mRho.V5.mER.hOr47a was a gift from Bill Hansson (Addgene plasmid # 126472 ; http://n2t.net/addgene:126472 ; RRID:Addgene_126472)

  • For your References section:

    Optimization of Insect Odorant Receptor Trafficking and Functional Expression Via Transient Transfection in HEK293 Cells. Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, Lavista-Llanos S. Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062. 10.1093/chemse/bjz062 PubMed 31504297
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