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Addgene

Ubiquitin WT
(Plasmid #12647)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12647 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin - wild type
  • Alt name
    Ubiquitin C
  • Alt name
    UBCEP52
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    228
  • GenBank ID
    AAA72608.1
  • Entrez Gene
    UBC (a.k.a. HMG20)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a 76 amino acid synthetic ubiquitin insert used to determine the 3D structure of the UbcH5c/Ub noncovalent complex, as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ubiquitin WT was a gift from Rachel Klevit (Addgene plasmid # 12647 ; http://n2t.net/addgene:12647 ; RRID:Addgene_12647)

  • For your References section:

    A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE. Mol Cell. 2006 Mar 17. 21(6):873-80. 10.1016/j.molcel.2006.02.008 PubMed 16543155
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