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PurposeExpresses human AQP4 (isoform M23) as an EmGFP fusion protein in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA 6.2 C-EmGFP
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Backbone manufacturerInvitrogen
- Total vector size (bp) 6720
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAquaporin 4
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Alt nameAQP4 M23 isoform
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SpeciesH. sapiens (human)
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Entrez GeneAQP4 (a.k.a. MIWC, MLC4, WCH4, hAQP4)
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Tag
/ Fusion Protein
- EmGFP (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA6.2 EmGFP hAQP4(M23) was a gift from Markus Reindl (Addgene plasmid # 126464 ; http://n2t.net/addgene:126464 ; RRID:Addgene_126464) -
For your References section:
Complement activating antibodies to myelin oligodendrocyte glycoprotein in neuromyelitis optica and related disorders. Mader S, Gredler V, Schanda K, Rostasy K, Dujmovic I, Pfaller K, Lutterotti A, Jarius S, Di Pauli F, Kuenz B, Ehling R, Hegen H, Deisenhammer F, Aboul-Enein F, Storch MK, Koson P, Drulovic J, Kristoferitsch W, Berger T, Reindl M. J Neuroinflammation. 2011 Dec 28;8:184. doi: 10.1186/1742-2094-8-184. 10.1186/1742-2094-8-184 PubMed 22204662