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Addgene

pEGFP-N1 hMOG-alpha1-EGFP
(Plasmid #126463)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126463 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 5465
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myelin oligodendrocyte glycoprotein
  • Alt name
    MOG alpha-1 isoform
  • Species
    H. sapiens (human)
  • Entrez Gene
    MOG (a.k.a. BTN6, BTNL11, MOGIG2, NRCLP7)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV forward
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1 hMOG-alpha1-EGFP was a gift from Markus Reindl (Addgene plasmid # 126463 ; http://n2t.net/addgene:126463 ; RRID:Addgene_126463)
  • For your References section:

    Complement activating antibodies to myelin oligodendrocyte glycoprotein in neuromyelitis optica and related disorders. Mader S, Gredler V, Schanda K, Rostasy K, Dujmovic I, Pfaller K, Lutterotti A, Jarius S, Di Pauli F, Kuenz B, Ehling R, Hegen H, Deisenhammer F, Aboul-Enein F, Storch MK, Koson P, Drulovic J, Kristoferitsch W, Berger T, Reindl M. J Neuroinflammation. 2011 Dec 28;8:184. doi: 10.1186/1742-2094-8-184. 10.1186/1742-2094-8-184 PubMed 22204662