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PurposeExpression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126463 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Total vector size (bp) 5465
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMyelin oligodendrocyte glycoprotein
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Alt nameMOG alpha-1 isoform
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SpeciesH. sapiens (human)
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Entrez GeneMOG (a.k.a. BTN6, BTNL11, MOGIG2, NRCLP7)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1 hMOG-alpha1-EGFP was a gift from Markus Reindl (Addgene plasmid # 126463 ; http://n2t.net/addgene:126463 ; RRID:Addgene_126463) -
For your References section:
Complement activating antibodies to myelin oligodendrocyte glycoprotein in neuromyelitis optica and related disorders. Mader S, Gredler V, Schanda K, Rostasy K, Dujmovic I, Pfaller K, Lutterotti A, Jarius S, Di Pauli F, Kuenz B, Ehling R, Hegen H, Deisenhammer F, Aboul-Enein F, Storch MK, Koson P, Drulovic J, Kristoferitsch W, Berger T, Reindl M. J Neuroinflammation. 2011 Dec 28;8:184. doi: 10.1186/1742-2094-8-184. 10.1186/1742-2094-8-184 PubMed 22204662