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BARD1 26-327 pET28a
(Plasmid #12646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRCA1 binding partner for a Ring domain E3 Ub-ligase
  • Alt name
    BARD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    906
  • Entrez Gene
    BARD1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The BARD1 insert in this plasmid contains a K153E mutation, when compared to GenBank reference sequence NP_000456.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BARD1 26-327 pET28a was a gift from Rachel Klevit (Addgene plasmid # 12646 ; http://n2t.net/addgene:12646 ; RRID:Addgene_12646)

  • For your References section:

    A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE. Mol Cell. 2006 Mar 17. 21(6):873-80. 10.1016/j.molcel.2006.02.008 PubMed 16543155
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