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Addgene

pJHG004
(Plasmid #126455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5523
  • Total vector size (bp) 7053
  • Modifications to backbone
    Added a Kozak consensus sequence downstream of the NheI site. Switched neomycin resistance to hygromycin.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dTdT
  • Alt name
    terminal deoxynucleotidyl transferase
  • Alt name
    DNA nucleotidylexotransferase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1530
  • Mutation
    Added D343E and D345E to inactivate the enzyme.
  • GenBank ID
    NM_004088.3
  • Entrez Gene
    DNTT (a.k.a. TDT)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The inactivating D343E and D345E were identified by Yang et al., 1994, J Biol Chem. 1994 Apr 22;269(16):11859-68 and combined by Repasky et al., 2004, doi: 10.4049/jimmunol.172.9.5478. Please visit https://doi.org/10.1101/639120 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJHG004 was a gift from Chang Liu (Addgene plasmid # 126455 ; http://n2t.net/addgene:126455 ; RRID:Addgene_126455)

  • For your References section:

    Lineage tracing and analog recording in mammalian cells by single-site DNA writing. Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC. Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 10.1038/s41589-021-00769-8. Epub 2021 Mar 22. 10.1038/s41589-021-00769-8 PubMed 33753928
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