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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET151D topo
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 4500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE3-Ring domain, Ub-ligase
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Alt nameBRCA1
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SpeciesH. sapiens (human)
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Insert Size (bp)912
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Entrez GeneBRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ cloning site topo (unknown if destroyed)
- 3′ cloning site topo (unknown if destroyed)
- 5′ sequencing primer Universal T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BRCA1 (1-304) was a gift from Rachel Klevit (Addgene plasmid # 12645 ; http://n2t.net/addgene:12645 ; RRID:Addgene_12645) -
For your References section:
A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE. Mol Cell. 2006 Mar 17. 21(6):873-80. 10.1016/j.molcel.2006.02.008 PubMed 16543155
