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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12644 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerstratagene
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUbch5c - Ubiquitin conjugating enzyme
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Alt nameUbcH5c
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SpeciesH. sapiens (human)
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Insert Size (bp)444
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Entrez GeneUBE2D3 (a.k.a. E2(17)KB3, UBC4/5, UBCH5C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer universal T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UbcH5c S22R pET28a was a gift from Rachel Klevit (Addgene plasmid # 12644 ; http://n2t.net/addgene:12644 ; RRID:Addgene_12644) -
For your References section:
A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE. Mol Cell. 2006 Mar 17. 21(6):873-80. 10.1016/j.molcel.2006.02.008 PubMed 16543155