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UbcH5c WT pET28a
(Plasmid #12643)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12643 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UbcH5c - Ubiquitin conjugating enzyme
  • Alt name
    UbcH5c
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    444
  • Entrez Gene
    UBE2D3 (a.k.a. E2(17)KB3, UBC4/5, UBCH5C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer universal T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UbcH5c WT pET28a was a gift from Rachel Klevit (Addgene plasmid # 12643 ; http://n2t.net/addgene:12643 ; RRID:Addgene_12643)

  • For your References section:

    A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE. Mol Cell. 2006 Mar 17. 21(6):873-80. 10.1016/j.molcel.2006.02.008 PubMed 16543155
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