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Addgene

pcDNA-Cas9-T2A-STOP-TdT
(Plasmid #126425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5523
  • Total vector size (bp) 11253
  • Modifications to backbone
    Added a Kozak consensus sequence downstream of the NheI site. Hygromycin resistance has replaced neomycin.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-T2A-STOP-TdT
  • Alt name
    Cas9
  • Alt name
    STOP-TdT
  • Species
    H. sapiens (human); Streptococcus pyogenes
  • Insert Size (bp)
    5730
  • Mutation
    alanine-6 of TdT has been changed to a STOP codon
  • GenBank ID
    NM_004088.3
  • Entrez Gene
    DNTT (a.k.a. TDT)
  • Promoter pCMV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    George Church; Cas9 is from Addgene plasmid 41815.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/639120 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Cas9-T2A-STOP-TdT was a gift from Chang Liu (Addgene plasmid # 126425 ; http://n2t.net/addgene:126425 ; RRID:Addgene_126425)

  • For your References section:

    Lineage tracing and analog recording in mammalian cells by single-site DNA writing. Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC. Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 10.1038/s41589-021-00769-8. Epub 2021 Mar 22. 10.1038/s41589-021-00769-8 PubMed 33753928
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