CaMKIV-DCTK75E
(Plasmid #126423)

• Purpose: A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
• Depositing Lab: Douglas Black
• Publication: Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi: 10.1038/35073593.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6423
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: calcium/calmodulin-dependent protein kinase IV kinase-dead mutant
• Alt name: CAMK IV kinase-dead mutant
• Alt name: CAMK 4 kinase-dead mutant
• Species: H. sapiens (human)
• Insert Size (bp): 989
• Mutation: changed lysine 75 to glutamic acid
• GenBank ID: NM_001744
• Entrez Gene: CAMK4 (a.k.a. CaMK IV, CaMK-GR, CaMKIV, caMK)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry