CaMKIV-dCT
(Plasmid
#126422)
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PurposePlasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6423
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCalcium/calmodulin-dependent protein kinase type IV
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Alt nameCAMK IV
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Alt nameCAMK 4
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SpeciesH. sapiens (human)
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Insert Size (bp)995
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GenBank IDNM_001744
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Entrez GeneCAMK4 (a.k.a. CaMK IV, CaMK-GR, CaMKIV, caMK)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CaMKIV-dCT was a gift from Douglas Black (Addgene plasmid # 126422 ; http://n2t.net/addgene:126422 ; RRID:Addgene_126422) -
For your References section:
A CaMK IV responsive RNA element mediates depolarization-induced alternative splicing of ion channels. Xie J, Black DL. Nature. 2001 Apr 19;410(6831):936-9. doi: 10.1038/35073593. 10.1038/35073593 PubMed 11309619