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Addgene

pcDNA3-mSmo-NQ4-V5
(Plasmid #126416)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126416 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    m-Smoothened
  • Species
    M. musculus (mouse)
  • Mutation
    Smo glycosylation site NQ4
  • Entrez Gene
    Smo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-mSmo-NQ4-V5 was a gift from Stacey Ogden (Addgene plasmid # 126416 ; http://n2t.net/addgene:126416 ; RRID:Addgene_126416)

  • For your References section:

    Functional Divergence in the Role of N-Linked Glycosylation in Smoothened Signaling. Marada S, Navarro G, Truong A, Stewart DP, Arensdorf AM, Nachtergaele S, Angelats E, Opferman JT, Rohatgi R, McCormick PJ, Ogden SK. PLoS Genet. 2015 Aug 20;11(8):e1005473. doi: 10.1371/journal.pgen.1005473. eCollection 2015 Aug. 10.1371/journal.pgen.1005473 PubMed 26291458
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