pUAS-attB V5 dDispatched delta CS HA
(Plasmid
#126412)
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PurposeExpression of V5 dDispatched Delta CS HA in Drosophila cells lines
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126412 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUAS
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert named-Dispatched1
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SpeciesD. melanogaster (fly)
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MutationDisp1 Cleavage site
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Entrez Genedisp (a.k.a. Dmel_CG2019, CG2019, Disp, Dmel\CG2019, anon-WO0146227.1)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAS-attB V5 dDispatched delta CS HA was a gift from Stacey Ogden (Addgene plasmid # 126412 ; http://n2t.net/addgene:126412 ; RRID:Addgene_126412) -
For your References section:
Cleavage activates dispatched for Sonic Hedgehog ligand release. Stewart DP, Marada S, Bodeen WJ, Truong A, Sakurada SM, Pandit T, Pruett-Miller SM, Ogden SK. Elife. 2018 Jan 23;7. pii: 31678. doi: 10.7554/eLife.31678. 31678 [pii] PubMed 29359685