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Addgene

pCDNA3 V5 mDispatched WT 2x HA
(Plasmid #126410)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126410 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    m-Dispatched1
  • Species
    M. musculus (mouse)
  • Mutation
    V5 iserted between A106 and A107
  • Entrez Gene
    Disp1 (a.k.a. 1190008H24Rik, DispA)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 V5 mDispatched WT 2x HA was a gift from Stacey Ogden (Addgene plasmid # 126410 ; http://n2t.net/addgene:126410 ; RRID:Addgene_126410)

  • For your References section:

    Cleavage activates dispatched for Sonic Hedgehog ligand release. Stewart DP, Marada S, Bodeen WJ, Truong A, Sakurada SM, Pandit T, Pruett-Miller SM, Ogden SK. Elife. 2018 Jan 23;7. pii: 31678. doi: 10.7554/eLife.31678. 31678 [pii] PubMed 29359685
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