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Addgene

pAc V5 dDispatched WT HA
(Plasmid #126405)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126405 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    d-Dispatched1
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    disp (a.k.a. Dmel_CG2019, CG2019, Disp, Dmel\CG2019, anon-WO0146227.1)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc V5 dDispatched WT HA was a gift from Stacey Ogden (Addgene plasmid # 126405 ; http://n2t.net/addgene:126405 ; RRID:Addgene_126405)

  • For your References section:

    Cleavage activates dispatched for Sonic Hedgehog ligand release. Stewart DP, Marada S, Bodeen WJ, Truong A, Sakurada SM, Pandit T, Pruett-Miller SM, Ogden SK. Elife. 2018 Jan 23;7. pii: 31678. doi: 10.7554/eLife.31678. 31678 [pii] PubMed 29359685
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