LNCX TbetaRII DN
(Plasmid #12640)

• Depositing Lab: Rik Derynck
• Publication: Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Backbone

• Vector backbone: LNCX
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TGF-beta receptor II
• Species: H. sapiens (human)
• Insert Size (bp): 600
• Mutation: Dominant negative: truncated cytoplasmically
• Entrez Gene: TGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, tbetaR-II)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: HpaI (destroyed during cloning)
• 5′ sequencing primer: LNCX

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry