LPCX Smad3 deltaSSVS
(Plasmid #12639)

• Depositing Lab: Rik Derynck
• Publication: Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Backbone

• Vector backbone: LPCX
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Smad3
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: Last 4 aa's deleted.
• Entrez Gene: SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: HpaI (destroyed during cloning)
• 5′ sequencing primer: LNCX

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

LPCX was made by replacing the neo gene of LNCX with the puro gene.