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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX 2T
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmad3
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Alt nameMADR3
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SpeciesH. sapiens (human)
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Insert Size (bp)1275
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Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for details.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX Smad3 was a gift from Rik Derynck (Addgene plasmid # 12630 ; http://n2t.net/addgene:12630 ; RRID:Addgene_12630) -
For your References section:
Receptor-associated Mad homologues synergize as effectors of the TGF-beta response. Zhang Y, Feng X, We R, Derynck R. Nature. 1996 Sep 12. 383(6596):168-72. 10.1038/383168a0 PubMed 8774881
Map uploaded by the depositor.
