pTEI129
(Plasmid
#126210)
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PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepICH47751
- Total vector size (bp) 8347
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Vector typePlant Expression ; Binary, Module 1
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameccdB Cassette (BsaI)
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SpeciesE.coli
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Insert Size (bp)1458
Gene/Insert 2
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Gene/Insert nameGFP11x7 (5 aa linker)
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Alt nameGFP11 M3
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SpeciesSynthetic
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Insert Size (bp)450
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byHuang Lab via Addgene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTEI129 was a gift from Ralf-Bernd Klösgen (Addgene plasmid # 126210 ; http://n2t.net/addgene:126210 ; RRID:Addgene_126210) -
For your References section:
Targeting specificity of nuclear-encoded organelle proteins with a self-assembling split-fluorescent protein toolkit. Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB. J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 10.1242/jcs.230839. 10.1242/jcs.230839 PubMed 31085714