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p1149-pCSDest-AcrIIA4-mCherry-miR122BS
(Plasmid #126205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSDest2
  • Backbone size w/o insert (bp) 4954
  • Total vector size (bp) 5215
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    codon-optimized AcrIIA4
  • Insert Size (bp)
    261
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG/NLS (C terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATTTAGGTGACACTATAGA
  • 3′ sequencing primer TAATACGACTCACTATAG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p1149-pCSDest-AcrIIA4-mCherry-miR122BS was a gift from Erik Sontheimer (Addgene plasmid # 126205 ; http://n2t.net/addgene:126205 ; RRID:Addgene_126205)

  • For your References section:

    Tissue-restricted Genome Editing in vivo Specified by MicroRNA-repressible Anti-CRISPR Proteins. Lee J, Mou H, Ibraheim R, Liang SQ, Liu P, Xue W, Sontheimer EJ. RNA. 2019 Aug 22. pii: rna.071704.119. doi: 10.1261/rna.071704.119. 10.1261/rna.071704.119 PubMed 31439808
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