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pBbS12a-RFP
(Plasmid #126175)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBb
  • Backbone size w/o insert (bp) 3590
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow bacterial cultures in 50µM Ampicillin
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RFP
  • Species
    Synthetic
  • Insert Size (bp)
    678
  • Promoter J23151

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Grow bacterial cultures in 50 micromolar Ampicillin. Cloning method was In-Fusion.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbS12a-RFP was a gift from Nigel Scrutton (Addgene plasmid # 126175 ; http://n2t.net/addgene:126175 ; RRID:Addgene_126175)

  • For your References section:

    SelProm: A Queryable and Predictive Expression Vector Selection Tool for Escherichia coli. Jervis AJ, Carbonell P, Taylor S, Sung R, Dunstan MS, Robinson CJ, Breitling R, Takano E, Scrutton NS. ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399. 10.1021/acssynbio.8b00399 PubMed 30870592
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