GEV 1
(Plasmid #12615)

• Depositing Lab: John Louis
• Publication: Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5443
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GB1 domain of protein G as N terminal tag, with Bcl2 as insert
• Alt name: GB1 domain of protein G
• Alt name: Bcl-2
• Alt name: Bcl2 (aa 82-204)
• Species: M. musculus (mouse)
• Insert Size (bp): 369
• Entrez Gene: Bcl2 (a.k.a. Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik)
• Tags / Fusion Proteins:
  • G protein (N terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe I (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In GEV1, a noncleavable linker (Pro-Gly-Gly-Pro-Ala-Ser) is encoded between the GBl domain and the N-terminus of the protein of interest. Genes can be inserted into this vector using Nhe I and Xho I cloning sites, and, if no stop codons are introduced at the 5' end of an insert, the vector encodes a C-terminal poly His-tag.

Also see: "A rapid method to attain isotope labeled small soluble peptides for NMR studies", Journal of Biomolecular NMR, Vol 26, No 3, July 2003.