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Addgene

pTEI177
(Plasmid #126077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH47742
  • Total vector size (bp) 8643
  • Vector type
    Plant Expression ; Binary, Module 1

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ccdB Cassette (BsaI)
  • Species
    E.coli
  • Insert Size (bp)
    1458

Gene/Insert 2

  • Gene/Insert name
    GFP1-10
  • Alt name
    GFP1-10 OPT
  • Species
    Synthetic
  • Insert Size (bp)
    666
  • Tag / Fusion Protein
    • linker (GTGGGGS) (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTEI177 was a gift from Ralf-Bernd Klösgen (Addgene plasmid # 126077 ; http://n2t.net/addgene:126077 ; RRID:Addgene_126077)

  • For your References section:

    Targeting specificity of nuclear-encoded organelle proteins with a self-assembling split-fluorescent protein toolkit. Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB. J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 10.1242/jcs.230839. 10.1242/jcs.230839 PubMed 31085714
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