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Addgene

pLaNGo11-1
(Plasmid #126053)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126053 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAGM4723
  • Total vector size (bp) 11484
  • Vector type
    Plant Expression ; Binary

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    chloroplastic FNR transit peptide
  • Alt name
    FNR (tp)
  • Species
    Spinacia oleracea
  • Insert Size (bp)
    834
  • GenBank ID
    M86349.1
  • Tag / Fusion Protein
    • GFP1-10 (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    ccdB cassette (BsaI)
  • Species
    E. coli
  • Insert Size (bp)
    1458

Gene/Insert 3

  • Gene/Insert name
    GFP11
  • Alt name
    GFP11 M3
  • Insert Size (bp)
    72

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Huang Lab via Addgene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLaNGo11-1 was a gift from Ralf-Bernd Klösgen (Addgene plasmid # 126053 ; http://n2t.net/addgene:126053 ; RRID:Addgene_126053)
  • For your References section:

    Targeting specificity of nuclear-encoded organelle proteins with a self-assembling split-fluorescent protein toolkit. Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB. J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 10.1242/jcs.230839. 10.1242/jcs.230839 PubMed 31085714