pLaNGo11-1
(Plasmid
#126053)
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PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 126053 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAGM4723
- Total vector size (bp) 11484
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Vector typePlant Expression ; Binary
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namechloroplastic FNR transit peptide
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Alt nameFNR (tp)
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SpeciesSpinacia oleracea
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Insert Size (bp)834
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GenBank IDM86349.1
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Tag
/ Fusion Protein
- GFP1-10 (C terminal on insert)
Gene/Insert 2
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Gene/Insert nameccdB cassette (BsaI)
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SpeciesE. coli
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Insert Size (bp)1458
Gene/Insert 3
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Gene/Insert nameGFP11
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Alt nameGFP11 M3
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Insert Size (bp)72
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byHuang Lab via Addgene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLaNGo11-1 was a gift from Ralf-Bernd Klösgen (Addgene plasmid # 126053 ; http://n2t.net/addgene:126053 ; RRID:Addgene_126053) -
For your References section:
Targeting specificity of nuclear-encoded organelle proteins with a self-assembling split-fluorescent protein toolkit. Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB. J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 10.1242/jcs.230839. 10.1242/jcs.230839 PubMed 31085714