pCMV-AR-R405S
(Plasmid #126051)

• Purpose: Expresses human androgen receptor (R405S) in mammalian cells
• Depositing Lab: Elizabeth Wilson
• Publication: Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-15. doi: 10.1074/jbc.M111.336081. Epub 2012 Feb 13.

Backbone

• Vector backbone: pCMV5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AR
• Alt name: androgen receptor
• Species: H. sapiens (human)
• Mutation: R405S
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The human AR N-terminal glutamine repeat may undergo minor expansion or contraction during bacterial propagation. It may be advisable to limit the time of bacterial growth and to verify glutamine repeat length using Sanger sequencing. Minor changes in AR N-terminal glutamine repeat length should not significantly alter AR activity.