pFA6a-TurboID-3MYC-natMX6 (pFB1434)
(Plasmid #126050)

• Purpose: For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker
• Depositing Lab: François Bachand
• Publication: Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249. doi: 10.1242/jcs.232249.

Backbone

• Vector backbone: pFA6a
• Total vector size (bp): 4993
• Vector type: PCR-based C-terminal tagging
• Selectable markers: NatMX6 (select with Nourseothricin)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TurboID

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (unknown if destroyed)
• 3′ cloning site: PacI (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The TurboID insert was originally described in: Efficient proximity labeling in living cells and organisms with TurboID. Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry