PB_tre_dCas9_VP160
(Plasmid #126031)

• Purpose: PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion
• Depositing Lab: Mauro Calabrese
• Publication: Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118.

Backbone

• Vector backbone: piggyBac cargo vector
• Backbone manufacturer: System Biosciences
• Total vector size (bp): 11825
• Vector type: Mammalian Expression, CRISPR ; piggyBac
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: dCas9 fusion with VP160
• Alt name: dCas9-VP160
• Insert Size (bp): 4623
• Mutation: D10A, H840A (catalytically inactive Cas9)
• Promoter: TRE

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGGAAAAGGCCTCCACGG

Gene/Insert 2

• Gene/Insert name: Hygromycin resistance
• Insert Size (bp): 1008
• Promoter: EF1 alpha

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: SpeI (destroyed during cloning)
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • PB_tre_dCas9_VP160.ape
• A portion of this plasmid was derived from a plasmid made by: dCas9-VP160 from Addgene #48225 (Cheng et al., 2013)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/448803v1 for BioRxiv preprint