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Addgene

PB_rtTA_BsmBI
(Plasmid #126028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggyBac cargo vector
  • Backbone manufacturer
    System Biosciences
  • Backbone size (bp) 8660
  • Modifications to backbone
    rtTA3-IRES-Neo cassette cloned from pSLIK-Neo (Addgene#25735). U6 expression cassette from pX330 (Addgene#42230) with dual BsmBI sites was cloned into the PacI site upstream of the rtTA3-IRES-Neo cassette.
  • Vector type
    Mammalian Expression, CRISPR ; piggyBac
  • Promoter U6
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/448803v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB_rtTA_BsmBI was a gift from Mauro Calabrese (Addgene plasmid # 126028 ; http://n2t.net/addgene:126028 ; RRID:Addgene_126028)

  • For your References section:

    A piggyBac-based toolkit for inducible genome editing in mammalian cells. Schertzer MD, Thulson E, Braceros KCA, Lee DM, Hinkle ER, Murphy RM, Kim SO, Vitucci EM, Calabrese JM. RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118. 10.1261/rna.068932.118 PubMed 31101683
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