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pHRW-CRY2olig-VHH(GFP)
(Plasmid #126023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHRW
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRY2 PHRolig-VHH(GFP)
  • Species
    A. thaliana (mustard weed)
  • Mutation
    CRY2 E490G
  • Promoter Hsp70
  • Tag / Fusion Protein
    • RFP (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CRY2 from Arabidopsis thaliana was obtained from pCMV-SNAP-CRY2-VHH(GFP) (Addgene 58370) as described in the methods of Osswald et al., 2019. CRY2olig-VHH(GFP) was generated by site-directed mutagenesis. Construct is heat shock inducible.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHRW-CRY2olig-VHH(GFP) was a gift from Eurico Morais-de-Sá (Addgene plasmid # 126023 ; http://n2t.net/addgene:126023 ; RRID:Addgene_126023)

  • For your References section:

    Light-Induced Protein Clustering for Optogenetic Interference and Protein Interaction Analysis in Drosophila S2 Cells. Osswald M, Santos AF, Morais-de-Sa E. Biomolecules. 2019 Feb 12;9(2). pii: biom9020061. doi: 10.3390/biom9020061. 10.3390/biom9020061 PubMed 30759894
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