pBabe-Puro-RhoA FLARE.sc Biosensor
(Plasmid #12602)

• Depositing Lab: Klaus Hahn
• Publication: Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Backbone

• Vector backbone: pBabe-Puro-CMV
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RhoA Biosensor
• Alt name: 6xHis-RBD-CFP-Linker-Citrine-RhoA WT
• Alt name: RhoA
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: blunt (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pBABE-5

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!

Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html